A Model Peptide Reveals Insights into the Interaction of Human Hemopexin with Heme

نویسندگان

چکیده

Abstract Under hemolytic conditions, toxic heme is scavenged by hemopexin. Recently, the heme-binding properties of hemopexin have been reassessed, which revealed a K D ~ 0.32 nM as well stoichiometry one to two molecules binding A 66mer hemopexin-derived peptide that spans over three motifs was used verify earlier suggested heme-recruiting mechanism. Herein, we employed spectroscopic and computational methods substantiate hypothesis more than molecule analyze mode. Both, peptide, were found bind in mixed penta- hexacoordinated states, strongly indicates follows distinct criteria increases rigidity peptide-heme complex. Additional silico molecular dynamics simulations support these experimental findings and, thus, contribute our understanding basis heme-hemopexin interaction. This analysis provides further details for consideration biomedical applications.

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ژورنال

عنوان ژورنال: International Journal of Peptide Research and Therapeutics

سال: 2022

ISSN: ['1573-3904', '1573-3149']

DOI: https://doi.org/10.1007/s10989-022-10441-x